Biology 311 Developmental Mechanisms and Embryology
Laboratory Exercise: Structure Function Relationships in the Nervous System
December 2, 4, 5, 1997
Dr. Jacobs


This laboratory will explore several basic mechanisms involved in information processing in the nervous system. The cricket cercal sensory system will be used as a model to study these mechanisms.

  1. The relationship between the structural characteristics of individual neurons and their functional properties.
  2. How an ensemble of neurons forms a topographic map.
  3. How a topographic map represents information about sensory stimuli.

Introduction:

Structure-function relationships in the nervous system:
One of the most basic concepts involved in the organization of neural systems is the relationship between the anatomical structure of neurons and their functional properties. These structure function relationships can be studied at many different levels. At a macroscopic level, the location a particular neuron in the brain, determines what behaviors that neuron will participate in. For example, a motor neuron in the spinal cord is involved in controlling the movements of a particular muscle. A ganglion cell in the retina is involved in processing visual stimuli. At the microscopic level the location of the dendritic and axonal processes of an neuron within a neural structure, determines the inputs it receives from other neurons and the neurons it transmits information to. At the ultrastructural level the distribution of synapses and ion channels over branches of a neuron determine how that cell integrates information. The structure of a neuron, at all of these levels, is tightly linked to its functional properties.

Topographic maps in sensory systems:
Sensory systems are organized anatomically to represent certain features of the sensory environment in the central nervous system. This anatomical organization is called a neural map. Your brain contains many such maps of the external world, including maps of the visual world, the auditory world and your body surface. These neural maps have evolved as an efficient means of organizing the massive amount of sensory information that comes in to the brain in a way that is easy to access and analyze by the rest of the nervous system. For example, in the auditory system there exists a map of sound frequency. Neurons sensitive to low sound frequencies are located at one location in the map and those sensitive to higher frequencies are located at another location of the map. Cells sensitive to intermediate frequencies are located in a stepwise fashion through the map. Thus there is a continuous representation of sound frequency within the auditory system. In general, cells that are sensitive to similar stimuli (like the same sound frequency) are located near each other in a map, and cells sensitive to different stimuli are spatially separated in the map.

Topographic mapping of receptor direction in the cricket cercal sensory system:
In this laboratory you will examine the relationship between the functional properties of individual sensory receptors, sensory interneurons and their location within a neural map. The system you will study is an insect sensory system, the cricket cercal sensory system. This system allows the animal to detect air currents in its local environment, such as those produced by an approaching predator. The system analyzes the direction and velocity of the air currents, which tells the cricket the location of the approaching predator. With this information, the cricket can then turn away from the predator and make its escape.

Goal:

The goal of this laboratory exercise is to understand the structure function relationships between neurons within a neural map. There are four different experiments in the exercise:
  1. to measure the length and distribution of a sample of mechanosensory hairs on the cercus.
  2. to understand how an ensemble of neurons forms a topographic map
  3. to determine how the topographic map represents information about sensory stimuli and
  4. to understand how primary sensory interneurons extract information from the map.

This laboratory will use anatomical data from the cricket cercal system and anatomical reconstruction and imaging techniques to address these questions.

The images that will be used in this laboratory have been assembled into a series of web pages. For each activity, use one or more sets of images (slides) to answer the questions on your lab handout.